7-Hydroxymitragynine is a potent alkaloid found in kratom with analgesic properties. This study examines its extraction, composition, and pharmacological effects. High-performance liquid chromatography (HPLC) confirmed its concentration, while biological tests evaluated efficacy. Results indicate strong opioid receptor binding, with potential applications in pain management. However, concerns about dependency and regulation remain. Further research is needed to establish its safety and viability as a dietary supplement.